United Kingdom (UK)
Recombinant monoclonal antibody to MS4A4A. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3F2.
UniProt Accession Number of Target Protein: Q96JQ5
Alternative Name(s) of Target: Four-span transmembrane protein 1; human membrane-spanning 4-domains subfamily A member 4A; CD20 antigen-like 1; (MS4A4A)-GFP; MS4A4; 4SPAN1; MS4A
Immunogen: This antibody was generated by immunizing C57BL/6 mice against mouse cell line 300-19 expressing transfected human membrane-spanning 4-domains subfamily A member 4A (MS4A4A)-GFP.
Specificity: This antibody is specific for human extracellular MS4A4A. The 3F2 and 4H2 (Ab01373) mAb clones completely cross-block one another indicating that they might bind to the same epitope or proximal epitopes (Sanyal et al., 2017; pmid: 28303902). MS4A (membrane-spanning 4-domain family, subfamily A) is a large family of proteins that includes at least 26 members in mouse and humans. Flanked by amino- and carboxyl- cytoplasmic regions, MS4A family members contain four highly conserved transmembrane domains connected by short extracellular and cytoplasmic loops. MS4A4A is likely to be a component of oligomeric cell surface complexes involved in signal transduction in diverse cell lineages.
Application Notes: 3F2 is recommended for the detection and analysis of expression of MS4A4A. Importantly, MS4A4A has been shown to be a marker for M2 macrophages and plasma cells and to be overexpressed in multiple myelomas, suggesting a potential role in targeting alternatively activated macrophages such as tumor-associated macrophages, and in the treatment of multiple myeloma and mantle cell lymphoma (Sanyal et al., 2017; pmid: 28303902). The antibody 3F2 can be successfully used for the detection of MS4A4A-expressing cells via flow cytometry (Sanyal et al., 2017; pmid: 28303902). Finally, specificity of this antibody was additionally confirmed via immunoprecipitation of lysates of BJAB MS4A4A-HA cells (Sanyal et al., 2017; pmid: 28303902).
Antibody first published in:
Sanyal et al. MS4A4A: a novel cell surface marker for M2 macrophages and plasma cells. Immunol Cell Biol. 2017 Aug;95(7):611-619. doi: 10.1038/icb.2017.18. Epub 2017 Mar 17. PMID:28303902
Note on publication:
This article describes the generation and characterization of the antibody 3F2.
Immunofluorescence staining of fixed K562 cells with anti-MS4A4A antibody 3F2 (Ab01375) Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 3F2 (Ab01375-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01375-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.