United Kingdom (UK)
Recombinant monoclonal antibody to MS4A4A. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 4H2.
UniProt Accession Number of Target Protein: Q96JQ5
Alternative Name(s) of Target: Four-span transmembrane protein 1; human membrane-spanning 4-domains subfamily A member 4A; CD20 antigen-like 1; (MS4A4A)-GFP; MS4A4; 4SPAN1; MS4A
Immunogen: This antibody was generated by immunizing C57BL/6 mice against mouse cell line 300-19 expressing transfected human membrane-spanning 4-domains subfamily A member 4A (MS4A4A)-GFP.
Specificity: This antibody is specific for human extracellular MS4A4A. The 4H2 and 3F2 (Ab01375) mAb clones completely cross-block one another indicating that they might bind to the same epitope or proximal epitopes (Sanyal et al., 2017; pmid: 28303902). MS4A (membrane-spanning 4-domain family, subfamily A) is a large family of proteins that includes at least 26 members in mouse and humans. Flanked by amino- and carboxyl- cytoplasmic regions, MS4A family members contain four highly conserved transmembrane domains connected by short extracellular and cytoplasmic loops. MS4A4A is likely to be a component of oligomeric cell surface complexes involved in signal transduction in diverse cell lineages.
Application Notes: 4H2 is recommended for the detection and analysis of expression of MS4A4A. Importantly, MS4A4A has been shown to be a marker for M2 macrophages and plasma cells and to be overexpressed in multiple myelomas, suggesting a potential role in targeting alternatively activated macrophages such as tumor-associated macrophages, and in the treatment of multiple myeloma and mantle cell lymphoma (Sanyal et al., 2017; pmid: 28303902). The antibody 4H2 can be successfully used for the detection of MS4A4A-expressing cells via flow cytometry (Sanyal et al., 2017; pmid: 28303902). Finally, specificity of this antibody was additionally confirmed via immunoprecipitation of lysates of BJAB MS4A4A-HA cells (Sanyal et al., 2017; pmid: 28303902).
Antibody first published in:
Sanyal et al. MS4A4A: a novel cell surface marker for M2 macrophages and plasma cells. Immunol Cell Biol. 2017 Aug;95(7):611-619. doi: 10.1038/icb.2017.18. Epub 2017 Mar 17. PMID:28303902
Note on publication:
This article describes the generation and characterization of the antibody 4H2.