Anti-MUC1 [PAM4]

Description:

Recombinant monoclonal antibody to MUC1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma PAM4.

Clone Name:

PAM4

Published Application(s):

therapeutic, WB, ELISA, FC, IHC

Published Species Reactivity:

Human

Products based on this clone:

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Ab02454-23.0 Anti-MUC1 [PAM4]
Human
Rabbit IgG
Purified
In Stock

Ab02454-1.1 Anti-MUC1 [PAM4]
Human
Mouse IgG1
Purified
Ships in 4-5 weeks

Ab02454-10.0 Anti-MUC1 [PAM4]
Human
Human IgG1
Purified
Ships in 4-5 weeks

Ab02454-10.3 Anti-MUC1 [PAM4]
Human
Human IgG1
Fc Silent™
Purified
Ships in 4-5 weeks

UniProt Accession Number of Target Protein: P15941

Alternative Name(s) of Target: Mucin-1; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut-reactive urinary mucin; PUM; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial membrane antigen; EMA; Tumor-associated mucin; CD227
Immunogen: The original antibody was raised by immunization of mice with the PAM4 antigen.

Specificity: This antibody is specific for the PAM4 domain of the human MUC1 protein.

Application Notes: This antibody was used for immunohistochemistry on normal adult tissues and neoplastic tissues. This yielded no to weak staining in normal tissue and stronger staining in 85% of the neoplastic tissue (US7238786). ELISA was performed on blood serum of healthy humans, pancreatic cancer patients, pancreatitis patients, ovarian cancer patients and breast cancer patients (US7238786). This antibody was used to treat mice that were given the CaPanl tumor. The animals showed regression of tumor (US7238786). ELISA was performed on blood sera of cancer patients using this antibody (Gold et al, 2005; pmid:16344318). Immunohistochemistry was performed on 63 PanIN and 36 IPMN lesions using this antibody (Gold et al, 2007; pmid:18094420). Flow cytometry was performed on the cell lines: RPMI 8226, U266, MC/CAR JJN-3, KMS12-BM, KMS 12-PE, DUL4, MUC1, breast carcinoma cell lines, MCF7, MDA-MB468, T47D, ZR-75-30, and a pancreatic carcinoma cell line, CaPan-1. This was done using this antibody (Burton et al, 1999; pmid:10541345). A western blot was performed using this antibody (Burton et al, 1999; pmid:10541345).

Antibody first published in: Gold et al. PAM4-reactive MUC1 is a biomarker for early pancreatic adenocarcinoma. Clin Cancer Res. 2007 Dec 15;13(24):7380-7. PMID:18094420
Note on publication: The expression of the PAM4-reactive MUC1 in the noninvasive precursor lesions, pancreatic intraepithelial neoplasia (PanIN) and intraductal papillary mucinous neoplasia (IPMN) was examined.

Flow cytometry analysis of MCF7 cells using Ab02454-23.0. Click on image for more details.

Flow cytometry using the Anti-MUC1 antibody PAM4 (Ab02454). Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of PAM4 (Ab02454-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

IF staining of MCF7 cells using Ab02454-23.0. Click on image for more details.

Immunofluorescence staining of MCF7 cells with anti-MUC1 (Ab02454) PAM4 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of PAM4 (Ab02454-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab02454-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.