United Kingdom (UK)
Recombinant monoclonal antibody to CD4. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma GK1.5.
UniProt Accession Number of Target Protein: P06332
Alternative Name(s) of Target: T-cell surface glycoprotein CD4; Cd4; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3
Immunogen: The antibody was raised by immunizing a Lewis rat with an i.v. injection of 30 x 10' Ficoll-Hypaque-purified cells from the cloned CTL line 243/2.5
Specificity: This antibody is specific for CD4. CD4 is an integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.
Application Notes: The effect of this antibody on the PFC response was measured by treating spleen cells from PC-FGG-primed BALB/c mice with this antibody (Dialynas et al, 1983; pmid:6415170). Immunofluorescence was preformed preformed on spleen cells from PC-FGG-primed BALB/c mice with this antibody (Dialynas et al, 1983; pmid:6415170). An flow cytometry experiment was preformed on spleen cells from PC-FGG-primed BALB/c mice with this antibody (Dialynas et al, 1983; pmid:6415170). The effect of this antibody on antigen-specific release of IL2 by mouse T cell hydridomas (Wilde et al, 1983; pmid:6195255). ELISA was preformed with this antibody on calf fetal serum (Wilde et al, 1983; pmid:6195255). The effect of the antibody on antigen-specific binding by T cell hybridoma AODH-3.4 was determined (Wilde et al, 1983; pmid:6195255). ELISA was preformed on murine CD4 using this antibody (Freise et al, 2017; pmid:27966069). The biodistribution of this antibody in mice was determined (Freise et al, 2017; pmid:27966069). Flow cytometry was preformed on blood, Thymus, lymph nodes, and spleen cells from mice with this antibody (Freise et al, 2017; pmid:27966069). Mouse C57BL/6 splenocytes were used in a proliferation assay with this antibody (Freise et al, 2017; pmid:27966069). Mouse Blood, spleen, and lymph node lymphocyte were used for flow cytometry using this antibody (Rice et al, 1995; pmid:7539027). Multiple organs from mice were used for immunohistochemistry using this antibody (Rice et al, 1995; pmid:7539027). Flow cytomic detection was used to detect T cell depletion using this antibody (Rashid et al, 1992; pmid:1538121).
Antibody first published in:
Dialynas et al. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule J Immunol. 1983 Nov;131(5):2445-51. PMID:6415170
Note on publication:
In the paper the antibody was raised by immunizing a Lewis rat with an i.v. injection of 30 x 10' Ficoll-Hypaque-purified cells from the cloned CTL line 243/2.5.
Immunofluorescence staining of mouse splenocytes with anti-CD4 antibody GK1.5 (Ab02561). Immunofluorescence analysis of paraformaldehyde-fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of GK1.5 (Ab02561-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, Ab02561-23.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.