United Kingdom (UK)
Recombinant monoclonal antibody to NKG2A. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma Z199.
UniProt Accession Number of Target Protein: P26715
Alternative Name(s) of Target: CD159a; NKG2-A; NKG2a; KLRC1; NK cell receptor A; CD159 antigen-like family member A; NKG2-A/NKG2-B type II integral membrane protein; NKG2-A/B-activating NK receptor
Specificity: This antibody binds the human NKG2a. It can also recognize and bind the CD94/NKG2a heterodimer formed by the covalent association of CD94 with the NKG2a molecule. The CD94/NKG2a heterodimer acts as an inhibitory natural killer cell receptor and binds nonclassical MHC class I protein, HLA-E. NKG2a is found mainly on NK cells and on subsets of CD8+ cells.
Application Notes: This antibody was reported to be a non-competitive antagonist of human CD94/NKG2A receptor expressed on lymphocytes, since it inhibited the function of CD94/NKG2A. Z199 can block the function of human CD94/NKG2A, and specifically induce killing of cells by CD94/NKG2A-restricted lymphocytes in a concentration-dependent fashion (US8796427). This antibody was used to study the covalent interactions between CD94 and NKG2A, which together form the inhibitory natural killer cell receptor (PMID: 9045931). This antibody was used to study the binding interactions of HLA-E (wild-type) tetramer to CD94/NKG2A or CD94/NKG2C expressing cells by flow cytometry (PMID: 14971033). This antibody was used to test the cytotoxic activity of NKL cells against tumor cells. NKL cells were tested for their ability to lyse the indicated targets in a 51Cr release assay, this antibody was found to enhance NK lysis (PMID: 21969815).
Antibody first published in:
Carretero et al. The CD94 and NKG2-A C-type lectins covalently assemble to form a natural killer cell inhibitory receptor for HLA class I molecules. Eur J Immunol. 1997 Feb;27(2):563-7. PMID:9045931
Note on publication:
Describes the use of this antibody to study the covalent interactions between CD94 and NKG2-A.
Immunofluorescence staining of K562 cells with anti-NKG2A antibody Z199 (Ab02978). Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix™ plus cover-slips stained with the chimeric rabbit IgG version of Z199 (Ab02978-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, Ab02978-23.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.