United Kingdom (UK)
Recombinant monoclonal antibody to NKp46. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma Bab281.
UniProt Accession Number of Target Protein: O76036
Alternative Name(s) of Target: Natural cytotoxicity triggering receptor 1; Lymphocyte antigen 94 homolog; NK cell-activating receptor; Natural killer cell p46-related protein; NK-p46; hNKp46; CD335
Immunogen: The original antibody was generated by immunizing Balb/c mice with recombinant human NKp46 protein.
Specificity: The antibody is specific for human NKp46. The antibody cross-reacts with macaque NK46 but does not cross-react with mouse NKp46. NKp46 is a receptor expressed by NK cells.
Application Notes: The antibody was used to immunoprecipitate NKp46 molecules from NK and COS-7 cells (Pessino et al., 1998; PMID 9730896 and Cantoni et al. 1999; PMID: 10049942). NK cells were analyzed for NKp46 surface expression by immunofluorescence and Flow Cytometry analysis using the antibody (Sivori et al., 1999; PMID: 10359120). The antibody binding to NKp46 promoted an increase of cytolytic activity in NK cells (Sivori et al., 1997; PMID: 9314561 and Sivori et al., 1999; PMID: 10359120).
Antibody first published in:
Sivori et al. p46, a novel natural killer cell-specific surface molecule that mediates cell activation J Exp Med. 1997 Oct 6;186(7):1129-36. PMID:9314561
Note on publication:
The paper describes the generation and characterization of the antibody.
Flow cytometry using anti-NKp46 antibody Bab281 (Ab03165). Human peripheral blood leukocytes were stained with the anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of Bab281 (Ab03165-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow cytometer.
Immunofluorescence staining of human peripheral blood leucocytes with anti-NKp46 antibody Bab281 (Ab03165). Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leucocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of Bab281 (Ab03165-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, Ab03165-23.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.