Anti-NMDA Receptor [17]

Description:

Recombinant monoclonal antibody to NMDA Receptor. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 17.

Clone Name:

Published Application(s):

crystallography

Published Species Reactivity:

Rat

Products based on this clone:

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Ab00900-1.1 Anti-NMDA Receptor [17]
Rat
Mouse IgG1
Purified
Ships in 4-5 weeks

Ab00900-1.6 Anti-NMDA Receptor [17]
Rat
Mouse Fab fragment
His-Tagged
Purified
Ships in 4-5 weeks

Ab00900-8.1 Anti-NMDA Receptor [17]
Rat
Rat IgG2b
Purified
Ships in 4-5 weeks

Ab00900-8.4 Anti-NMDA Receptor [17]
Rat
Rat IgG2b
Fc Silent™
Purified
Ships in 4-5 weeks

Ab00900-23.0 Anti-NMDA Receptor [17]
Rat
Rabbit IgG
Purified
In Stock

UniProt Accession Number of Target Protein: Q00960

Alternative Name(s) of Target: NMDAR; NMDA-R; Glutamate receptor
Immunogen: Monoclonal antibodies that bind rat GluN2B ATD were obtained by immunizing mice with the purified intact GluN1-GluN2B NMDA receptors. IgGs were purified from hybridoma cell culture supernatant by Protein-A Sepharose. Fab fragments have been prepared by papain proteolysis followed by re-chromatographing onto Protein-A Sepharose to remove Fc.

Specificity: Reacts specifically with rat GluN2B ATD.

Application Notes: This antibody has been used for the crystallisation of the NMDA receptor in the absence of an allosteric inhibitor at 2.9A (Tajima 2016).

Antibody first published in: Tajima et al. Activation of NMDA receptors and the mechanism of inhibition by ifenprodil Nature. 2016 May 2; 534(7605): 63–68 PMID:27135925
Note on publication: Describes the lack of high resolution structures representing the active state of the NMDA receptor.

IF staining of C6 cells using Ab00900-23.0. Click on image for more details.

Immunofluorescence staining of C6 cells with anti-NMDA Receptor (Ab00900) 17 Immunofluorescence analysis of paraformaldehyde fixed C6 cells stained with the chimeric rabbit IgG version of 17 (Ab00900-23.0) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00900-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Flow cytometry analysis of C6 cells using Ab00900-23.0. Click on image for more details.

Flow cytometry using the Anti-NMDA Receptor antibody 17 (Ab00900). Paraformaldehyde fixed C6 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of 17 (Ab00900-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

IF staining of C6 cells using Ab00900-23.0. Click on image for more details.