United Kingdom (UK)
Recombinant monoclonal antibody to non-structural protein 4. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody Nb41.
UniProt Accession Number of Target Protein: Q9YN02
Alternative Name(s) of Target: NSP4; Replicase polyprotein 1ab; ORF1ab polyprotein
Immunogen: An adult male bactrian camel was immunized subcutaneously with the purified Nsp4-NHis recombinant protein. A camel VHH library was then constructed and panned against Nsp4.
Specificity: The antibody is specific for Nsp4.
Application Notes: The specificity of the original format of the antibody was confirmed by indirect ELISA analysis. MARC-145 cell lines expressing nanobodies were generated and infected with PRRSV strain SD16. At 24 h post-infection the antibody inhibited infectious virus release by about 99%. Further, the antibody protected MARC-145 cells from any virus-induced cytopathic effect and fully blocked PRRSV replication (Liu et al. 2016; PMID: 27010387).
Antibody first published in:
Liu et al. Intracellularly expressed nanobodies against non-structural protein 4 of porcine reproductive and respiratory syndrome virus inhibit virus replication Biotechnol Lett. 2016 Jul;38(7):1081-8. PMID:27010387
Note on publication:
The original paper describes the generation and characterization of a set of antibodies by phage display method.