United Kingdom (UK)
Recombinant monoclonal antibody to Norovirus GII.4. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody 12B10.
Immunogen: A phage-displayed antibody library was generated from peripheral blood of healthy persons. The original antibody was isolated by screened using purified virus-like particles (VLPs) of strain Narita 104 (r104, genogroup II, genotype 4).
Specificity: The antibody is specific for Norovirus GII.4. It shows intra-genogroup cross-reactivity to genotypes 1, 4, 6, and 7 of genogroup II.
Application Notes: The specificity of the original format of the antibody was confirmed by ELISA analysis. The blocking activity of the antibody against the interaction of homotypic VLPs with histo-blood group antigens were also assessed as an alternative to neutralization assay. The antibody prevented the binding of r104 to histo-blood group antigens that had been reported to bind r104.
Antibody first published in:
Higo-Moriguchi et al. Isolation of cross-reactive human monoclonal antibodies that prevent binding of human noroviruses to histo-blood group antigens J Med Virol. 2014 Apr;86(4):558-67. PMID:24026909
Note on publication:
The original paper identified a panel of human monoclonal antibodies targeting norovirus.