United Kingdom (UK)
Recombinant monoclonal antibody to Norovirus P domain. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from phage display Nano-27.
UniProt Accession Number of Target Protein: Q83884
Immunogen: An alpaca was injected with norovirus GII.10 virus-like particles (VLPs), and a phage display library constructed. Nano-27 was selected from this phage display library.
Specificity: Nano-27 is specific for GII.10 strain of Norovirus, and does not show cross-reactivity with GI.1, GI.11, GII.1, GII.2, GII.4 2006 and 2012, GII.10, GII.12, or GII.17 strains.
Application Notes: The binding specificity of Nano-27 for the P domain of Norovirus strain GII.10 was confirmed using ELISA (Koromyslova et al, 2017). Using ITC the Kd of Nano-27 binding to the P domain of GII.10 was estimated as 9.50x10^-9 M (Koromyslova et al, 2017). The structure of Nano-27 in complex with G11.10 P domain was also obtained (Koromyslova et al, 2017). In neutralisation assays Nano-27 did not inhibit the binding of GII.10 to A-type saliva, B-type saliva, or pig gastric mucin (Koromyslova et al, 2017).
Antibody first published in:
Koromyslova et al, 2017. Nanobodies targeting norovirus capsid reveal functional epitopes and potential mechanisms of neutralization. PLoS Pathog. 2017 Nov 2;13(11):e1006636 PMID:29095961
Note on publication:
Describes the isolation of Nano-27 from a phage display library and its characterisation through ELISA, ITC, and crystallography.