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- Ab02404-23.0 Anti-Notch 1 [WC613]
- Human
- Rabbit IgG
- Purified
- In Stock
- Ab02404-1.1 Anti-Notch 1 [WC613]
- Human
- Mouse IgG1
- Purified
- Ships in 4-5 weeks
Recombinant monoclonal antibody to Notch 1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma WC613.
UniProt Accession Number of Target Protein: P46531
Alternative Name(s) of Target: Notch1; hN1; TAN-1; Neurogenic locus notch homolog protein 1; Translocation-associated notch protein TAN-1
Immunogen: The original antibody was isolated panning against phage display libraries generated from BMV (bone marrow), CS (spleen) and DP47 (frame work based CS) cells.
Specificity: This antibody recognizes and binds the ligand-binding region (LBD) of Notch 1.
Application Notes: This antibody is capable of immunoprecipitating Notch 1 but not Notch 4. The binding affinity of this antibody towards Notch1 was checked using ELISA. The binding of this antibody to full length Notch 1 overexpressed on HEK cells was confirm using flow cytometry. Flow cytometry was also used to study the expression of Notch 1 on cancer cell lines like LS-1034, TALL-1, BxPC3, and Colo_205. The antagonistic activity of WC613 was strongly dependent on the particular ligand-expressing cell line used to activate Notch1. This antibody competes with DLL4 for binding to Notch1. This antibody is capable of inhibiting Jag2 mediated Notch1 signalling with an EC50 value of 5±3 nM. This antibody completely inhibited Hes1 transactivation (PMID: 20161710).
Antibody first published in:
Aste-Amézaga et al. Characterization of notch1 antibodies that inhibit signaling of both normal and mutated notch1 receptors. PLoS One. (2010); 5(2): e9094. PMID:20161710
Note on publication:
Describes the generation and characterization of this antibody.