United Kingdom (UK)
Recombinant monoclonal antibody to NP. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody JS16.
UniProt Accession Number of Target Protein: P0DTC9
Alternative Name(s) of Target: NP; NC; Protein N; Nucleocapsid protein; SARS-CoV Protein N; SARS-CoV Nucleocapsid protein; SARS Coronavirus; SARS-CoV-2; SARS CoV 2; 2019-nCoV
Immunogen: A combinatorial fragment of antigen-binding (Fab) antibody phage library based on peripheral blood-derived from five coronavirus disease 2019 (COVID-19) infected donors was constructed. The antibody was obtained and identified by panning with purified NP.
Specificity: The antibody is specific for NP.
Application Notes: The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody detected the recombinant NP by western blot analysis. Surface plasmon resonance assay was performed to evaluate the binding specificity and affinity between antibodies and purified NP (KD in the sub-nM range). The antibody was used together with JS08 in sandwich ELISA (detection limit below 0.78ng/mL) (Zhang et al, 2020; PMID:33281824).
Antibody first published in:
Zhang et al. Development of Patient-Derived Human Monoclonal Antibodies Against Nucleocapsid Protein of Severe Acute Respiratory Syndrome Coronavirus 2 for Coronavirus Disease 2019 Diagnosis Front Immunol. 2020 Nov 13;11:595970. PMID:33281824
Note on publication:
The paper describes the generation and characterization of the antibody.