Anti-NS1 [ZKA35]

Name: Anti-NS1 [ZKA35]
SKU: Ab01036
Availability: InStock

Description:

Recombinant monoclonal antibody to NS1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the B-cell clone ZKA35.

Made with Antibody Sequences licensed from Humabs Biomed SA.

Clone Name:

ZKA35

Published Application(s):

Blockade-of-Binding ELISA, BOB ELISA, Competition ELISA, ELISA

Published Species Reactivity:

Zika Virus

Product based on this clone:

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Ab01036-10.0 Anti-NS1 [ZKA35]
Zika Virus
Human IgG1
HRP conjugate, Purified
In Stock

Alternative Name(s) of Target: ZIKV NS-1; non-structural protein 1; non structural protein 1
Immunogen: Antibody ZKA35 was isolated from peripheral blood mononuclear cell (PBMC) and blood plasma collected from a ZIKV-infected patient.

Specificity: This antibody binds to the Zika virus NS1 protein at a site not targeted by antibodies cross-reactive with Dengue virus (DENV1 -4) or other flaviviruses such as West Nile Virus or Yellow Fever Virus.

Application Notes: This antibody can be used to specifically detect Zika virus as it is not cross-reactive with other flaviviridae. Balmaseda and coworkers (PMID: 28716913) used this antibody to develop a blockade-of-binding (BOB) assay able to discriminate immune responses to Zika infection from infections by other flaviviruses. In this assay, binding of ZKA35 to an NS1-antigen coated plate is blocked by Zika-specific antibodies found in positive patient serum, whilst sera from patients infected with other flaviviruses will be unable to do so as they do not contain competitive antibodies. This assay is an alternative it RT-PCR based methods, which suffer from a narrow time window during which viral RNA is detectable in bodily fluids. We exclusively offer this antibody as a horseradish peroxidase (HRP) conjugate for easy use in BOB ELISA assays (Ab01036-10.0-BOB). Unlabeled ZKA35 (ZKA35 Ab01036-10.0-CTL) can be used as a positive control. At a concentration of 5 ug/ml, unlabeled ZKA35 fully blocks binding of ZKA35-HRP.We therefore offer this HRP-conjugated antibody alongside a 40 ug vial of unconjugated control. Please refer to Ab01036-10.0-BOC or individually as Ab01036-10.0-CTL. A suggested protocol can be found here. For each user and batch, we recommend to confirm the EC70 of ZKA35-HRP binding to ZIKV NS1 prior to use with samples. Please refer to Balmaseda et al. 2018 (PMID: 29305550 ) for further examples of the use of this antibody and its positive control.

Antibody first published in: Balmaseda et al. Antibody-based assay discriminates Zika virus infection from other flaviviruses Proc Natl Acad Sci U S A. 2017 Aug 1;114(31) PMID:28716913
Note on publication: Describes the development of the BOB-ELISA assay and characterisation of the specificity of this assay for Zika virus infection.

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