United Kingdom (UK)
Recombinant monoclonal antibody to Nsp9. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody Nb6.
UniProt Accession Number of Target Protein: Q8B912
Alternative Name(s) of Target: nonstructural protein 9; PRRSV; Replicase polyprotein 1ab; ORF1ab polyprotein
Immunogen: A Bactrian camel was immunized with recombinant Nsp9 protein and a VHH library was constructed. The antibody was isolated by panning against Nsp9.
Specificity: The antibody is specific for Nsp9 of PRRSV (strains SD-16, JXA1, GD-HD, and VR2332). Nsp9 possesses RNA-dependent RNA polymerase (RdRp) activity that is essential for genome replication and transcription.
Application Notes: MARC-145 cell lines stably expressing Nb6 were established and infected with PRRSV strain SD-16. Virus infection was strongly suppressed in MARC-145-Nb6 as shown by IFA. Further, the antibody could inhibit PRRSV replication (Liu et al., 2015; PMID: 26525739).
Antibody first published in:
Liu et al. An intracellularly expressed Nsp9-specific nanobody in MARC-145 cells inhibits porcine reproductive and respiratory syndrome virus replication Vet Microbiol. 2015 Dec 31;181(3-4):252-60. PMID:26525739
Note on publication:
The original paper describes the generation and characterization of the antibody