- Ab01323-1.1 Anti-Nucleophosmin [NA24]
- Mouse IgG1
- In Stock
- Ab01323-23.0 Anti-Nucleophosmin [NA24]
- Rabbit IgG
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- Ab01323-10.0 Anti-Nucleophosmin [NA24]
- Human IgG1
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Recombinant monoclonal antibody to Nucleophosmin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma NA24.
UniProt Accession Number of Target Protein: P06748
Alternative Name(s) of Target: NPM; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin; B23; NPM1; Nucleolar protein NO38
Immunogen: This antibody was raised by immunising BALB/C mice with GST fusion protein containing the N-terminal part of nucleophosmin fused to 14 amino acid of ALK protein.
Specificity: This antibody is specific towards nucleophasmin which is a widely expressed phosphorylated protein involved in various processes, such as ribosome assebly and transport, cell proliferation, protein chaperoning and centrosome duplication. Aberrations involving nucleophasmin are found in multiple conditions, for instance in a form of non-Hodgkin lymphoma or acute promyelocytic leukemia.
Application Notes: Mutations associated with nucleophasmin are present in varous conditions and this antibody has proven useful in different experiments investigating their molecular underpinnings. One group used NE24 antibody to perform immunohistochemical staining confirming that anaplastic large-cell lymphoma involves expression of mutated NPM-ALK fusion (Cordell et al., 1999). Finally, alterations of nucleophasmin expression were found in many cancers where this antibody might be utilised to analyse them. For example, Gimenez and colleagues (2010) performed immunohistochemical stainings and Western blot analyses to demonstrate aberrations in nucleophasmin expression in brain cancer.
Antibody first published in:
Cordell et al. Detection of normal and chimeric nucleophosmin in human cells. Blood. 1999 Jan 15;93(2):632-42. PMID:9885226
Note on publication: This article describes generation and characterisation of NA24 antibody.
Immunofluorescence staining of HeLa cells using anti-Nucleophosmin antibody (Ab01323) NA24. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton and stained with the chimeric mouse IgG version of NA24 (Ab01323-1.1) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing nucleoli and nucleoplasm staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01323-1.1, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (Ab00178-1.1) followed by Alexa Fluor® 488
Flow-cytometry using the anti-Nucleophosmin antibody NA24 (Ab01323). HeLa cells were fixed using paraformaldehyde, permeabilised using 0.5% Triton and stained with anti-unknown IgG antibody (Ab00178-1.1; isotype control, black line) or the mouse IgG-chimeric version of NA24 (Ab01323-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Western Blot using anti-Nucleophosmin antibody NA24 (Ab01323). nuclear lysates of HeLa(A) and HEK293(B) cells (35µg protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of NA24 (Ab01323-1.1) at 0.001 µg/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Flow cytometry using the Anti-Nucleophosmin antibody NA24 (Ab01323). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-1.1; isotype control, black line) or the mouse IgG1 version of NA24 (Ab01323-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.