Anti-p120/catenin [2B12]

Name: Anti-p120/catenin [2B12]
SKU: Ab02695
Availability: InStock

Description:

Recombinant monoclonal antibody to p120/catenin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 2B12.

Clone Name:

2B12

Published Application(s):

IP, WB

Published Species Reactivity:

Chicken, Hamster, Rat, Human, Mouse

Products based on this clone:

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Ab02695-1.1 Anti-p120/catenin [2B12]
Chicken, Hamster, Rat, Human, Mouse
Mouse IgG1
Purified
Ships in 5-6 weeks

Ab02695-10.0 Anti-p120/catenin [2B12]
Chicken, Hamster, Rat, Human, Mouse
Human IgG1
Purified
Ships in 5-6 weeks

Ab02695-10.3 Anti-p120/catenin [2B12]
Chicken, Hamster, Rat, Human, Mouse
Human IgG1
Fc Silent™
Purified
Ships in 5-6 weeks

Ab02695-23.0 Anti-p120/catenin [2B12]
Chicken, Hamster, Rat, Human, Mouse
Rabbit IgG
Purified
Ships in 5-6 weeks

UniProt Accession Number of Target Protein: O60716

Alternative Name(s) of Target: Catenin delta-1; Cadherin-associated Src substrate; CAS; p120 catenin; p120(ctn); p120(cas)
Immunogen: The original antibody was generated immunizing A/J mice with tyrosine phosphoproteins purified from chicken embryonic fibroblasts (CEFs) expressing pp60/c-src 527F and 2A/527F.

Specificity: The antibody recognizes mouse, rat, hamster, human and chicken p120/catenin, but it does not cross react with the porcine and canine homologs. The epitope is located at amino acid residues 55‐101.

Application Notes: The antibody (IgG1) was used to immunoprecipitate tyrosine phosphoprotein P120. The antibody was used in Western blot (Kanner at al, 1990; PMID:2110361). The antibody was used to analyse the level of tyrosine phosphorylation of P120 in uninfected CE cells and cells expressing several oncogenic and nononcogenic src variants. The antibody was used to immunoprecipitate P120 from CE cell extracts, then the antibody was used in western blot (Kanner et al, 1991; PMID:1703631). The antibody was employed to perform immunocytochemical analysis in order to investigate the cellular localization of p120 on CMT 93/69 and MDCK cells (Staddon et al, 1995; PMID: 7615637). The antibody immunoprecipitated only CAS1 isoforms (Mo et al., 1996). The antibody was used for immunoprecipitation and Western blotting of pp120 from MDCK cell lysates (Dillon et al, 1998; PMID: 9422525).

Antibody first published in: Kanner et al. Monoclonal antibodies to individual tyrosine-phosphorylated protein substrates of oncogene-encoded tyrosine kinases Proc Natl Acad Sci U S A.1990 May;87(9):3328-32. PMID:2110361
Note on publication: The paper describes the generation and characterization of the antibody.

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