United Kingdom (UK)
Recombinant monoclonal antibody to p53 (a.a. 16-25). Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma X77.
UniProt Accession Number of Target Protein: P04637
Alternative Name(s) of Target: Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
Immunogen: The original mouse IgG1 version of this antibody was raised by immunizing mice against xenopus p53
Specificity: This antibody recognizes the human p73 protein as well as the human and xenopus p53 protein.
Application Notes: This original mouse IgG1 can be used to detect human p73 as well as human and xenopus p53. The reaction of this antibody with the two proteins was verified using immunoprecipitation and western blot (Le Bras et al., 2002; PMID: 11850851). The same group used indirect immunofluorescence to verify the specificity of the antibody (Le Bras et al., 2002; PMID: 11850851). Another group used the antibody with immunoprecipitation and immunoblotting to detect the accumulation of human p53 with increasing levels of calpastatin (Pariat et al., 1997; PMID: 9111352).
Antibody first published in:
Le Bras et al. Monoclonal antibodies raised against Xenopus p53 interact with human p73 Oncogene. 2002 Feb 14;21(8):1304-8 PMID:11850851
Note on publication:
Describes the characterization of the original X77 clone which was generated in the same paper
Western Blot using anti-p53 (a.a. 16-25) antibody X77 (Ab02776). A431 (0.0001µg/ml) cells nuclear lysate (35µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of X77 (Ab02776-23.0) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Immunofluorescence staining of A431 cells with anti-p53 (a.a. 16-25) (Ab02776) X77 Immunofluorescence analysis of A431 cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of X77 (Ab02776-23.0) (1:1000 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab02776-23.0, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (Ab00102-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.