Anti-PD-L1 [B3]

Name: Anti-PD-L1 [B3]
SKU: Ab00840
Availability: InStock

Description:

Recombinant monoclonal antibody to PD-L1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the alpaca peripheral lymphocytes B3.

Clone Name:

Published Application(s):

functional assay, in vivo, Block, ELISA, FC

Published Species Reactivity:

Mouse

Product based on this clone:

{{heading}}

Please Select

Ab00840-23.9 Anti-PD-L1 [B3]
Mouse
Rabbit IgG-Fc fusion
His-Tagged
Purified
In Stock

UniProt Accession Number of Target Protein: Q9EP7

Alternative Name(s) of Target: CD274; B7 Homolog 1; Programmed Cell Death 1 Ligand 1; PDCD1 Ligand 1; LDCD1LG1; PDCD1L1; B7H1; PDL1; Programmed Death Ligand 1; B7-H1; B7-H
Immunogen: The original antibody was generated by immunizing alpaca with the purified ectodomain of mouse PD-L1.

Specificity: This antibody binds mouse PD-L1 and does not cross react with human PD-L1. This protein acts as a ligand for the inhibitory receptor PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. It plays a critical role in induction and maintenance of immune tolerance to self.

Application Notes: This antibody could block the interaction between PD-L1 and PD-1 and was also reported to inhibit its binds with B7-1. In vitro competition experiments confirmed that B3 competes for binding with both PD-1 and the alternative ligand B7-1 (CD80) for PD-L1 binding. 18F-labeled antibody was used in vivo to study the expression of PD-L1 in brown adipose tissues using immuno PET-CT imaging (PMID: 28935898). This antibody was used for the identification of recombinant PD-L1 Fc fusion protein using ELISA. This antibody can recognize IFN-gamma inducible PD-L1 on multiple murine cancer cell lines (PMID: 18173375). This antibody augments in vitro cytotoxicity by OT-I cells against B16-OVA or by melanoma-specific TRP1 CD8 T cells cultured with B16 melanoma cells (PMID: 24459675). In vivo, it delays the growth of B16 melanoma cells after vaccination with GVAX. It binds pancreatic cancer cell lines Panc02 and KPC in vitro, and ex vivo on cells from resected orthotopic tumors (PMID: 29459478). A fusion protein B3-IL2 prepared using this antibody and when used in combination with anti-melanoma antibody TA99 it showed tumor growth and prolonged survival compared to treatment with TA99 and anti–PD-L1 alone (PMID: 29459478).

Antibody first published in: Ingram et al. PD-L1 is an activation-independent marker of brown adipocytes. Nat Commun. 2017 Sep 21;8(1):647. PMID:28935898
Note on publication: The study evaluates the expression of PD-L1 in brown adipose tissues (BAT) using a radiolabeled camelid single chain antibody and PET-CT imaging.

Privacy Overview

This website uses cookies to improve your experience while you navigate through the website. Out of these, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. We also use third-party cookies that help us analyze and understand how you use this website. These cookies will be stored in your browser only with your consent. You also have the option to opt-out of these cookies. But opting out of some of these cookies may affect your browsing experience.

Necessary

Always Enabled

Necessary cookies are absolutely essential for the website to function properly. These cookies ensure basic functionalities and security features of the website, anonymously.

Cookie	Duration	Description
ASP.NET_SessionId	session	Issued by Microsoft's ASP.NET Application, this cookie stores session data during a user's website visit.
cookielawinfo-checkbox-advertisement	1 year	Set by the GDPR Cookie Consent plugin, this cookie is used to record the user consent for the cookies in the "Advertisement" category .
cookielawinfo-checkbox-analytics	11 months	This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Analytics".
cookielawinfo-checkbox-functional	11 months	The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Functional".
cookielawinfo-checkbox-necessary	11 months	This cookie is set by GDPR Cookie Consent plugin. The cookies is used to store the user consent for the cookies in the category "Necessary".
cookielawinfo-checkbox-others	11 months	This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Other.
cookielawinfo-checkbox-performance	11 months	This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Performance".
viewed_cookie_policy	11 months	The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. It does not store any personal data.

Analytics

Analytical cookies are used to understand how visitors interact with the website. These cookies help provide information on metrics the number of visitors, bounce rate, traffic source, etc.

Cookie	Duration	Description
_dc_gtm_UA-49047364-1	1 minute	No description
_ga	2 years	The _ga cookie, installed by Google Analytics, calculates visitor, session and campaign data and also keeps track of site usage for the site's analytics report. The cookie stores information anonymously and assigns a randomly generated number to recognize unique visitors.
_gcl_au	3 months	Provided by Google Tag Manager to experiment advertisement efficiency of websites using their services.
_gid	1 day	Installed by Google Analytics, _gid cookie stores information on how visitors use a website, while also creating an analytics report of the website's performance. Some of the data that are collected include the number of visitors, their source, and the pages they visit anonymously.
ai_session	30 minutes	This is a unique anonymous session identifier cookie set by Microsoft Application Insights software to gather statistical usage and telemetry data for apps built on the Azure cloud platform.
ai_user	1 year	A unique user identifier cookie, set by Microsoft Application Insights software, that enables counting of the number of users accessing the application over time.

Advertisement cookies are used to provide visitors with relevant ads and marketing campaigns. These cookies track visitors across websites and collect information to provide customized ads.

Cookie	Duration	Description
_uetsid	1 day	Bing Ads sets this cookie to engage with a user that has previously visited the website.
_uetvid	1 year 24 days	Bing Ads sets this cookie to engage with a user that has previously visited the website.
MUID	1 year 24 days	Bing sets this cookie to recognize unique web browsers visiting Microsoft sites. This cookie is used for advertising, site analytics, and other operations.
test_cookie	15 minutes	The test_cookie is set by doubleclick.net and is used to determine if the user's browser supports cookies.