United Kingdom (UK)
Recombinant monoclonal antibody to Pharynx. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma KT22.
Immunogen: The original antibody was generated by immunizing BALB/c mice with subtracted embryonic extract. Subtracted extracts were prepared by using a method called “antigen subtraction” which enriched antigens of low abundance in heterogeneous molecule mixtures by subtracting abundant antigens.
Specificity: This antibody recognized the apical side of the pharynx of Caenorhabditis elegans. Caenorhabditis elegans is a transparent nematode that lives in temperate soil environments.
Application Notes: This antibody was used for the staining of the apical region of the pharynx of Caenorhabditis elegans. The KT22 signal was detectable in the twofold embryo as a single line from the buccal cavity to the end of isthmus; at the pretzel stage, the KT22 stained three lines from the buccal cavity to the anterior isthmus, which appeared to merge into a single line in the posterior isthmus region. No KT22 signal was detected in the terminal bulb and pharyngeal–intestinal valve (PMID: 18498356).
Antibody first published in:
Takeda et al. Efficient production of monoclonal antibodies recognizing specific structures in Caenorhabditis elegans embryos using an antigen subtraction method. Genes Cells. 2008 Jul;13(7):653-65. PMID:18498356
Note on publication:
Describes the use of "antigen subtraction" method to enrich antigens of low abundance in heterogeneous molecule mixtures by subtracting abundant antigens. The paper also discloses generation of 35 mAbs against Caenorhabditis elegans that recognize specific structures, including P granules, muscles, the pharynx, and subsets of hypodermal cells; some of the mAbs revealed previously unreported cellular structures.