United Kingdom (UK)
Recombinant monoclonal antibody to Podocalyxin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma mAb 2A4.
UniProt Accession Number of Target Protein: O00592
Alternative Name(s) of Target: PC; PCLP-1; GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; 2A4
Immunogen: The original antibody was generated by immunizing BALB/C mice with purified human podocalyxin-glutathione S-transferase (GST) fusion protein containing the intracellular, transmembrane, and a part of the extracellular domain of human PCLP.
Specificity: The antibody is specific for human podocalyxin. The epitope recognized by the antibody is located on the intracellular domain of podocalyxin. Podocalyxin, a sialoglycoprotein, is thought to be the major constituent of the glycocalyx of podocytes in the glomerulus (Bowman's capsule) in the kidneys. It is a member of the CD34 family of transmembrane sialomucins.
Application Notes: The antibody recognized rabbit PCLP1 in human renal cortical sections on immunofluorescence. The antibody detected PCLP by western blot analysis. The antibody recognized and immunoprecipitated rat podocalyxin (Kershaw et al., 1997; PMID: 9188463). The antibody detected PCLP in HEV of tonsil by immunofluorescence analysis. The antibody stained HEV of paraffin-embedded samples of lymph node and appendix by immunohistochemistry (Sassetti et al., 1998; PMID: 9625756).
Antibody first published in:
Kershaw et al. Molecular cloning and characterization of human podocalyxin-like protein. Orthologous relationship to rabbit PCLP1 and rat podocalyxin. J Biol Chem. 1997 Jun 20;272(25):15708-14. doi: 10.1074/jbc.272.25.15708. PMID:9188463
Note on publication:
The original paper describes the generation and characterization of the original antibody.