Anti-porcine VCAM [3F4]

Name: Anti-porcine VCAM [3F4]
SKU: Ab01173
Availability: InStock

Description:

Recombinant monoclonal antibody to porcine VCAM. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3F4.

Clone Name:

3F4

Published Application(s):

Blocking, ELISA, FC

Published Species Reactivity:

Pig

Products based on this clone:

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Ab01173-1.1 Anti-porcine VCAM [3F4]
Pig
Mouse IgG1
Purified
In Stock

Ab01173-23.0 Anti-porcine VCAM [3F4]
Pig
Rabbit IgG
Purified
In Stock

UniProt Accession Number of Target Protein: F1S567

Alternative Name(s) of Target: pVCAM
Immunogen: This antibody was raised by immunising BALB/c mice with a recombinant secreted form of porcine VCAM.

Specificity: This antibody is specific for an epitope within domain 2 of porcine VCAM, which is expressed on activated endothelial cells. It des not cross-react with human VCAM.

Application Notes: The ability of this antibody to recognise porcine VCAM has been confirmed in ELISA analysis (Mueller et al, 1995). In FC analysis, this antibody reacts with porcine VCAM on TNF-a stimulated porcine aortic endothelial cells, but not to human VCAM on TNF-a stimulated human umbilical vein endothelial cells (Mueller et al, 1995). This antibody blocks VLA-4 dependent adhesion of peripheral blood human T cells to activated porcine aortic endothelial cells (Mueller et al, 1995). It also inhibits the binding of Ramos cells to a recombinant secreted form of porcine VCAM (Mueller et al, 1995).

Antibody first published in: Mueller et al. Porcine vascular cell adhesion molecule (VCAM) mediates endothelial cell adhesion to human T cells. Development of blocking antibodies specific for porcine VCAM Transplantation, 1995, Vol.60(11), pp.1299-306 PMID:8525525
Note on publication: Describes the original generation of this antibody, and its characterisation in ELISA and FC analysis.

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