United Kingdom (UK)
Recombinant monoclonal antibody to PorM. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the llama peripheral lymphocytes Nb30.
UniProt Accession Number of Target Protein: B2RLE8
Alternative Name(s) of Target: Por secretion system protein porM/gldM; T9SS component cytoplasmic membrane protein PorM
Immunogen: The original antibody was isolated from a nanobody phage-display naïve library generated from the peripheral blood of non-immunized llamas. The specific antibody was isolated by panning the library against periplasmic domain of PorM protein of Porphyromonas gingivalis.
Specificity: This antibody binds N-terminal part comprising amino acids from 225–516 of the PorM protein of Porphyromonas gingivalis. PorM is a membrane protein that is involved in the assembly of the type IX secretion system (T9SS) in Porphyromonas gingivalis, a major bacterial pathogen that is responsible for periodontal disease in humans.
Application Notes: The binding specificity of this antibody towards C-terminal part of the PorM protein was determined using ELISA. The affinity of the antibody was found to be Kd= 4.5 ± 1.0 nM (PMID: 28471361).
Antibody first published in:
Duhoo et al. Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis. Acta Crystallogr F Struct Biol Commun. 2017 May 1;73(Pt 5):286-293. PMID:28471361
Note on publication:
Describes the generation of nanobodies against PorM protein of Porphyromonas gingivalis.