United Kingdom (UK)
Recombinant monoclonal antibody to Potato virus Y NIa. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma SA2.
UniProt Accession Number of Target Protein: P22602
Alternative Name(s) of Target: PVY; PVY NIa; NIa; Potato virus Y; Genome polyprotein; Nuclear inclusion a; aphid-borne virus; 22-1; Hybridoma 22
Immunogen: The original antibody was generated by immunizing mice with semipurified fraction of potato virus Y (PVY) as antigen.
Specificity: This antibody bind the nuclear inclusion A (NIa) protein of the Potato virus Y (PVY). Potato virus Y is a monopartite, single stranded RNA virus, plant pathogen that infects mainly Solanaceous plants including, potato, tomato, pepper, tobacco and eggplant. There are multiple strains of the virus including the common strain, PVYO, which causes mosaic symptoms in most hosts. The necrotic strains PVYN and PVYNTN both cause a veinal necrosis on tobacco; PVYNTN also causes a tuber necrosis in certain potato varieties. This necrosis is referred to as potato tubers necrotic ringspot disease (PTNRD). In general, the necrotic strains tend to cause a more mild mosaic symptom than PVYO in potato. PVY is transmitted in a non-persistent manner by aphids.
Application Notes: This antibody was used for in situ localization of potato virus Y NIa protein using immunofluorescence staining. The staining showed the accumulation of the NIa protein in both nucleus and cytoplasm. The binding characterization of the antibody was done using ELISA (PMID: 16884787). In vivo, this antibody was able to detect different forms of the NIa protein including precursors and cleavage products and also able to inhibit the cleavage of the polyprotein detected in the semipurified NI (PMID: 11556707).
Antibody first published in:
Rouis et al. Inhibition of potato virus Y NIa activity: preparation of monoclonal antibody directed against PVY NI protein that inhibits cleavage of PVY polyprotein. Arch Virol. 2001 Jul;146(7):1297-306. PMID:11556707
Note on publication:
Describes the generation of antibodies directed against PVY NI protein of Potato virus Y.