United Kingdom (UK)
Recombinant monoclonal antibody to Profilin-1. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma mAbPRF1a (2-14D9).
UniProt Accession Number of Target Protein: Q42449
Alternative Name(s) of Target: Ara t 8; PRO1; Profilin-1; Profilin 1; Profilin; PRF1
Immunogen: This antibody was raised by immunising mice with a purified plant Profilin-1.
Specificity: This antibody recognizes Profilin-1 and does not corss-react with other isoforms. Profilins are actin-binding proteins involved in the processing of actin cytoskeleton.They are also recognized allergens.
Application Notes: MAbPRF1a antibody is recommended for the identification of Profilin-1 and for the distinguishment of the Profilin-1 from other profilin isovariants in Arabidopsis sp. (McKinney et al., 2001). Western blot analysis confirmed that MAbPRF1a antibody is specific to Profilin-1 and does not cross-react with other isovariants (Kandasamy et al., 2002). This antibody was also used in immunohistochemical analysis of the distribution of profillins in various part of a plant identifying Profilin-1 mostly in vegetative tissues (Kandasamy et al., 2002).
Antibody first published in:
McKinney et al. Small changes in the regulation of one Arabidopsis profilin isovariant, PRF1, alter seedling development. Plant Cell. 2001 May;13(5):1179-91. PMID:11340190
Note on publication:
This article describes the generation and characterization of the mAbPRF1a antibody.