United Kingdom (UK)
Recombinant monoclonal antibody to Protein OPG154. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 1G6.
UniProt Accession Number of Target Protein: P11258
Alternative Name(s) of Target: A27L; Protein A27; OPG154; VACWR150
Immunogen: The original antibody was raised by immunizing a BALB/c mouse with VACVWR.
Specificity: This antibody is specific for residues 21 to 40 of the A27 protein.
Application Notes: The original version of this antibody was used for an ELISA on recombinant VACV A27 protein. This antibody was used for an in vitro flow cytometry neutralization assay (Kaever et al., 2016; PMID:26889021, Kaever et al., 2014; PMID:25031354). It showed the ability to prevent 90% of virus from binding to Vero E6 cells. The structure of this antibody was determined using x-ray crystallography (Kaever et al., 2016; PMID:26889021).
Antibody first published in:
Kaever et al. Linear Epitopes in Vaccinia Virus A27 Are Targets of Protective Antibodies Induced by Vaccination against Smallpox J Virol. 2016 May 1; 90(9): 4334–4345. PMID:26889021
Note on publication:
The original paper generated and subsequently characterized 7 murine monoclonal antibodies (MAbs), which fell into 4 distinct epitope groups (groups I to IV)