United Kingdom (UK)
Recombinant monoclonal antibody to Protein OPG161. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma A27D7.
UniProt Accession Number of Target Protein: P68617
Alternative Name(s) of Target: A33R; OPG161; VACWR156; Protein A33; Extracellular enveloped virus (EEV)-s
Immunogen: The original antibody was raised by immunizing a mouse with VACV.
Specificity: This antibody is specific for a conformational epitope on A33 of the vaccinia virus.
Application Notes: The original version of this antibody was able to prevent vaccinia virus challange in vivo when tested in mice. Furthermore the ability of this antibody to neutralize the vaccinia virus. Finally the epitope of this antibody was determined using an ELISA (Matho et al., 2015; PMID:26325270). The original antibody was used for a binding competition assay on biotin-labeled DAM. Further surface plasmon resonance was performed on DAM using this antibody (Wang et al., 2024; PMID:38182667).
Antibody first published in:
Matho et al. Structural and Functional Characterization of Anti-A33 Antibodies Reveal a Potent Cross-Species Orthopoxviruses Neutralizer PLoS Pathog. 2015 Sep; 11(9): e1005148. PMID:26325270
Note on publication:
The original paper produced seven murine anti-A33 monoclonal antibodies by immunizing mice with live VACV.