Anti-pSYK [MIL81-1-8]

Description:

Recombinant monoclonal antibody to pSYK. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MIL81-1-8.

Clone Name:

MIL81-1-8

Published Application(s):

ICC, ELISA, IF, IHC

Published Species Reactivity:

Human

Products based on this clone:

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Ab02455-23.0 Anti-pSYK [MIL81-1-8]
Human
Rabbit IgG
Purified
Ships in 5-6 weeks

Ab02455-1.1 Anti-pSYK [MIL81-1-8]
Human
Mouse IgG1
Purified
Ships in 5-6 weeks

UniProt Accession Number of Target Protein: P43405

Alternative Name(s) of Target: Tyrosine-protein kinase SYK; Spleen tyrosine kinase; p72-Syk
Immunogen: The original antibody was raised by immunizing New Zealand White rabbits with a peptide covering residues 520-529 of hSYK and residues corresponding to tyrosine 525 and tyrosine 526 are phosphorylated.

Specificity: This antibody is specific for p-Y525-SYK.

Application Notes: ELISA was performed on GST-SYK-p using this antibody (WO2015164330). Western blot was performed with this antibody on GST-pSYK (IVK), and phosphatase- treated GST-pSYK (WO2015164330). AlphaLISA® luminescence proximity assay was performed on IVK and PPase treated recombinant GST-SYK samples using this antibody (WO2015164330). This antibody was used for immunofluorescence on HeLa cells transfected for 24 hr with lipid: DNA complex (WO2015164330). Immunocytochemistry was performed using this antibody on WSU-DLCL2 samples untreated or activated by Fab crosslinking (WO2015164330). Immunohistochemistry was performed on PHTX-95L (WO2015164330). This antibody was also used for immunohistochemistry on BLl xenograft tissue and several primary human tissue xenografts (PHTX-95L, OCI LY10) (WO2015164330).

Antibody first published in: Brake et al. Anti-psyk antibody molecules and use of same for syk-targeted therapy. WO2015164330 PMID:

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