United Kingdom (UK)
Recombinant monoclonal antibody to PV11. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody III20.
Immunogen: The original scFv antibody was selected from a human synthetic scFv phage display library by panning against the PV11 strain of the virus.
Specificity: The antibody binds the PV11 strain of the rabies virus.
Application Notes: The specificity of the scFv-Fc format of the antibody was confirmed by ELISA analysis. The scFv-Fc version of the antibody detected GPRV by western blot analysis. The scFv-Fc version of the antibody bound specifically to the membranes of PV11 infected Vero cells in an immunofluorescence assay. The scFv-Fc version of the antibody neutralized the PV11 strain in a standard in vivo neutralization assay where the virus was incubated with the scFv-Fc fragment before intracranial inoculation in mice (Ray et al., 2001; PMID: 11472431).
Antibody first published in:
Ray et al. Selection of single chain variable fragments (scFv) against the glycoprotein antigen of the rabies virus from a human synthetic scFv phage display library and their fusion with the Fc region of human IgG1 Clin Exp Immunol. 2001 Jul; 125(1): 94–101. PMID:11472431
Note on publication:
The original paper describes the generation and characterization of the antibody.