United Kingdom (UK)
Recombinant monoclonal antibody to Rad51B. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 1h3/13.
UniProt Accession Number of Target Protein: O15315
Alternative Name(s) of Target: DNA repair protein RAD51 homolog 2; R51H2; RAD51 homolog B; RAD51-like protein 1; REC2; RAD51L1; 1 H3/13
Immunogen: This antibody was raised against His-tagged recombinant human Rad51B.
Specificity: This antibody is specific against Rad51B (does not cross-react with Rad51C, Rad51D, Rad51, XRCC2, or XRCC3 in Western analysis). Rad51B is a paralog of Rad51 which is an E. coli RecA homologue. It is invloved in the homologous recombination repair (HRR) pathway which fixes double-stranded breaks.
Application Notes: WB: rad51b 1h3/13 (1H3) antibody was used to visualise Rad51B on Western blots in multiple experiments (e.g. after previous gel filtration of infected pf9 cells; after immunoprecipitation with different antibodies and others) in a study on the RAD51 paralogs' complexes and their role in genetic recombination (Masson et al., 2001).
Antibody first published in:
Masson et al. Identification and purification of two distinct complexes containing the five RAD51 paralogs. Genes Dev. 2001 Dec 15;15(24):3296-307. PMID:11751635
Note on publication:
This article describes the earliest use of rad51b 1h3/13 (1H3) antibody to detect Rad51B protein via Western blotting. However, the exact process of generation of this antibody is described in the following paper (where similar antibodies were generated): Masson, J. Y., Stasiak, A. Z., Stasiak, A., Benson, F. E., & West, S. C. (2001). Complex formation by the human RAD51C and XRCC3 recombination repair proteins. Proceedings of the National Academy of Sciences, 98(15), 8440-8446.