United Kingdom (UK)
Recombinant monoclonal antibody to Rad51C. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 2H11/6.
UniProt Accession Number of Target Protein: O43502
Alternative Name(s) of Target: DNA repair protein RAD51 homolog 3; R51H3; RAD51 homolog C; RAD51-like protein 2; BROVCA3; FANCO; MGC104277; RAD51-like protein 2; yeast RAD51 homolog 3; RAD51L2RAD51 (S. cerevisiae) homolog C; RAD51L2; 2H11
Immunogen: This antibody was raised by immunising mice with the denaturated recombinant human RAD51C-His10 protein which had been purified from 2.4 liters of E. coli FB810 recA− pLysS carrying pRAD51C-16b.
Specificity: This antibody recognises Rad51C in human, mouse, primate and yeast. Rad51C is a Rad51 paralog, which is a homologue of RecA (E. coli). It is involved in homologous recombination during DNA replication and repair.
Application Notes: WB: Rad51C 2H11/6 antibody was used to visualise Rad51C on Western blots in multiple experiments (Masson et al., 2001). IF/ICC: This antibody was also used to stain Rad51C in HEK293 cells during experiments aimed at determining the cellular localization of Rad51C (Bennett and Knight, 2005).
Antibody first published in:
Masson et al. Complex formation by the human RAD51C and XRCC3 recombination repair proteins. Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8440-6. PMID:11459987
Note on publication:
This article describes the detailed generation of this anti-Rad51C antibody as well as successfully applies it in Western analysis.