United Kingdom (UK)
Recombinant monoclonal antibody to Reticulon-1C . Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma RNL-4.
UniProt Accession Number of Target Protein: Q16799
Alternative Name(s) of Target: NSP-C; Reticulon-1; Neuroendocrine-specific protein
Immunogen: The original antibody was generated by immunizing a BALB/c mouse with a synthetic peptide encompassing the unique 20 N-terminal amino acid sequence of Reticulon-1C.
Specificity: The antibody is specific for reticulon-1C. It recognizes an epitope located within the first 20 amino acids of Reticulon-1C (NSP-C).
Application Notes: The specificity of the original format of the antibody for NSP-C was established in an ELISA assay. Immunofluorescence analysis of COS-1 cells, transfected with NSP-C cDNA, showed staining of the endoplasmic reticulum with the antibody. The antibody detected NSP-C by western blot analysis (Senden et al., 1996; PMID: 8900485). This antibody was used for immunohistochemistry for the detection of NSP-C on lung carcinoid, atypical carcinoid of the lung, SCLC and squamous cell carcinoma (Senden et al., 1997; PMID: 9227337).
Antibody first published in:
Senden et al. Neuroendocrine-specific protein C (NSP-C): subcellular localization and differential expression in relation to NSP-A Eur J Cell Biol. 1996 Mar;69(3):197-213. PMID:8900485
Note on publication:
The original paper describes the generation and characterization of the antibody.