United Kingdom (UK)
Recombinant monoclonal antibody to Rim ABC protein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma Rim 3F4.
UniProt Accession Number of Target Protein: P78363
Alternative Name(s) of Target: Retinal-specific ATP-binding cassette transporter
Immunogen: Rim ABC protein.
Specificity: The antibody binds to human, mouse and bovine Rim ABC-transporter. The epitope has the following amino acid sequence: 'PLHPRTAGASR'
Application Notes: The antibody binds to Retinal specific ABC-transporter, which is localised at the outer segment disk of rod and cone cells. The linear epitope (PLHPRTAGASR) can be used as a protein tag.
Antibody first published in:
Illing et al. 1997 The 220-kDa rim protein of retinal rod outer segments is a member of the ABC transporter superfamily. Journal of Biological Chemistry 1997; 272(5)10303-10310. PMID:9092582
Note on publication:
Describes the identification of a 220-kDa rim protein and the generation of a monoclonal antibody against it.
Flow cytometry using the Anti-Rim ABC protein antibody Rim 3F4 (Ab01563). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of Rim 3F4 (Ab01563-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Immunofluorescence staining of HeLa cells with anti-Rim ABC protein (Ab01563) Rim 3F4 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells stained with the chimeric rabbit IgG version of Rim 3F4 (Ab01563-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01563-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.