United Kingdom (UK)
Recombinant monoclonal antibody to RNase A. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the camel peripheral blood lymphocytes cAb-RN05.
UniProt Accession Number of Target Protein: P61823
Alternative Name(s) of Target: Ribonuclease pancreatic; RNASE1; RNase 1; RNaseA; RNS1 RN05
Immunogen: The original antibody was generated by immunizing a dromedary (Camelus dromedarius) with bovine RNase A. The affinity matured version of this antibody was generated using synthetic combinatorial libraries.
Specificity: This antibody binds bovine RNase A. RNase A is an endonuclease enzyme that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. It acts on single-stranded and double-stranded RNA.
Application Notes: This antibody binds bovine RNase with an affinity in the picomolar range. This affinity matured version of VHH antibody cAb-RN05 binds bovine RNase with an affinity of Kd= 180 pM (PMID: 17888451). 5′–3′ cUMP that binds into the active site of RNase A does not compete with cAb-RN05 for binding to RNase A in an ELISA (PMID: 10196124).
Antibody first published in:
Koide et al. Exploring the capacity of minimalist protein interfaces: interface energetics and affinity maturation to picomolar KD of a single-domain antibody with a flat paratope. J Mol Biol. 2007 Nov 2;373(4):941-53. PMID:17888451
Note on publication:
Describes the affinity maturation of a VVH antibody against bovine RNase.