Anti-RRM2 [SAIC-30E-7]

Description:

Recombinant monoclonal antibody to RRM2. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the B-cell cDNA library SAIC-30E-7.

Clone Name:

SAIC-30E-7

Published Application(s):

immunoMRM, ELISA

Published Species Reactivity:

Human

Products based on this clone:

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Ab00323-23.0 Anti-RRM2 [SAIC-30E-7]
Human
Rabbit IgG
Purified
In Stock

Ab00323-1.1 Anti-RRM2 [SAIC-30E-7]
Human
Mouse IgG1
Purified
Ships in 4-5 weeks

UniProt Accession Number of Target Protein: P31350

Alternative Name(s) of Target: RR2; R2; RR2M; ribonucleotide reductase regulatory subunit M2; Ribonucleoside-diphosphate reductase subunit M2
Immunogen: The KLH-conjugated peptide "DIQHWESLKPEER" derived from Ribonucleoside-diphosphate reductase subunit M2 (RRM2). RRM2 provides precursors required for DNA synthesis.

Specificity: Recognises human RRM2.

Application Notes: Original data characterizing this antibody may be found here .

Antibody first published in: Schoenherr et al. Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Mol Cell Proteomics PMID:25512614
Note on publication: Describes generation a panel of recombinant antibodies using synthetic peptides of human proteins as well as validation using ELISA and multiple reaction monitoring-mass spectrometry.

IF staining of HeLa cells using Ab00323-23.0. Click on image for more details.

Immunofluorescence staining of HeLa cells with anti-RRM2 (Ab00323) SAIC-30E-7 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-30E-7 (Ab00323-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nucleoli staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00323-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.