United Kingdom (UK)
Recombinant monoclonal antibody to RuvC. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma RuvC 5G9/3.
UniProt Accession Number of Target Protein: P0A814
Alternative Name(s) of Target: Crossover junction endodeoxyribonuclease RuvC; Holliday junction nuclease RuvC; Holliday junction resolvase RuvC; EC 3.1.22.4; 5G9
Immunogen: This antibody was raised against RuvC protein from E. coli.
Specificity: This antibody recognises E. coli RuvC.
Application Notes: RuvC 5G9/3 (5G9) antibody was initially used to inhibit RuvC from interacting in a complex with other proteins and therefore to demonstrate the importance of this complex' formation in the resolution of Holliday junctions (Eggleston et al., 1997). IP: This antibody was also used to pull down complexes containing RuvC to analyse the interactions of RuvC with RuvA and RuvB (Davies and West, 1998).
Antibody first published in:
Eggleston et al. In vitro reconstitution of the late steps of genetic recombination in E. coli. Cell. 1997 May 16;89(4):607-17. PMID:9160752
Note on publication:
This article describes experimets for which RuvC 5G9/3 (5G9) antibody was created. The paper reports application of this anti-RuvC antibody to potentially inhibit creation of the RuvABC complex showing importance of the interactions of RuvC with other proteins in resolution of Holliday complexes.