United Kingdom (UK)
Recombinant monoclonal antibody to SEB. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody 4PCL2.
UniProt Accession Number of Target Protein: P01552
Alternative Name(s) of Target: Staphylococcal enterotoxin B; Enterotoxin type B; entB; 2F6D9G2
Immunogen: The original antibody was generated by immunizing eight-week-old female BALB/c mice were immunized subcutaneously with SEB at 3-week intervals. Later on a phage display library was generated from the variable regions of the hybridoma and this clone was isolated by panning against SEB.
Specificity: This antibody recognizes and binds Staphylococcal enterotoxin B.
Application Notes: This antibody can be used for the immundection of SEB in a sample. This antibody was derived from the murine parent antibody called 2F6D9G2, after PCR cloning the variable region genes of the hybridoma to generate a phage display library and isolating the specific clone that showed reactivity towards SEB. The binding reactivity of the scFv clone towards SEB was determined using ELISA. The scFv clone binds SEB with a high affinity of 3.16 nM as determined by surface plasmon resonance (SPR). This scFv antibody was also capable of detecting SEB in a western blot assay (PMID: 20952642).
Antibody first published in:
Singh et al. Construction of a single-chain variable-fragment antibody against the superantigen Staphylococcal enterotoxin B. Appl Environ Microbiol. 2010 Dec;76(24):8184-91. PMID:20952642
Note on publication:
Describes the generation of an antibody against Staphylococcal enterotoxin B and further describes the generation of a murine phage display library containing single-chain variable-fragment (ScFv) antibody having binding affinity against SEB.