United Kingdom (UK)
Recombinant monoclonal antibody to sfGFP. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody NbsfGFP01.
UniProt Accession Number of Target Protein: P42212
Alternative Name(s) of Target: Superfolder green fluorescent protein; GFP; Green fluorescent protein
Immunogen: The original antibody was generated by immunizing an adult one-humped camel with recombinant superfolder green fluorescent protein (sfGFP). An immune nanobody library was generated from the peripheral blood of the immunized camel. The particular antibody was isolated by panning the library against sfGFP.
Specificity: This antibody is specific for superfolder green fluorescent protein which is a better-folding GFP version, with the following mutations: S30R, Y39N, F64L, S65T, F99S, N105T, Y145F, M153T, V163A, I171V and A206V.
Application Notes: The binding specificity of this nanobody towards sfGFP was determined using ELISA. This antibody can also be used of the identification of sfGFP via a western blot (PMID: 25085037).
Antibody first published in:
Twair et al. Characterization of camel nanobodies specific for superfolder GFP fusion proteins. Mol Biol Rep. 2014 Oct;41(10):6887-98. PMID:25085037
Note on publication:
Describes the generation of camelid nanobodies against superfolder GFP.