United Kingdom (UK)
Recombinant monoclonal antibody to Sialoadhesin. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3B11 (3B11/11).
UniProt Accession Number of Target Protein: A7LCJ3
Alternative Name(s) of Target: CD169; pSn; Sn; p210; Siglec-1; Sialic acid-binding Ig-like lectin 1
Immunogen: The original antibody was generated by immunizing BALB/c mice with porcine alveolar macrophages.
Specificity: This antibody recognizes and binds porcine sialoadhesin.
Application Notes: This antibody is recommended to be used as a tool in the study of porcine macrophage maturation and differentiation and for determining their heterogeneity both in normal and pathological conditions. This antibody can recognize porcine sialoadhesin in a western blot assay and an immunoprecipitation assay (PMID: 9151393). Tissue distribution of the antigen recognized by this mAb was analyzed by immunohistochemical staining of different tissues (PMID:19081005). 3B11 successfully binds alveolar macrophages and PAM cells in a flow cytometric analysis (PMID:19081005, 23785195). Porcine sialoadhesin has also been analyzed as potential antigen targeting receptors using mouse IgG as surrogate antigens (PMID:21557980). The mAb was also used in the study the role of sialoadhesin in porcine reproductive and respiratory syndrome virus infection using immunoflurosence and flow cytometric analysis (Liu et al, 2020)
Antibody first published in:
Bullido et al. Monoclonal antibodies specific for porcine monocytes/macrophages: macrophage heterogeneity in the pig evidenced by the expression of surface antigens. Tissue Antigens (1997); 49(4):403-13. PMID:9151393
Note on publication:
Describes the distribution of surface antigens on porcine monocytes and macrophages using monoclonal antibodies.