Anti-Sonic Hedgehog [5E1]

Description:

Recombinant monoclonal antibody to Sonic Hedgehog. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 5E1.

Clone Name:

5E1

Published Application(s):

Blocking, crystallisation, WB, ELISA, IHC

Published Species Reactivity:

Rat, Human

Products based on this clone:

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Ab01175-1.1 Anti-Sonic Hedgehog [5E1]
Rat, Human
Mouse IgG1
Purified
In Stock

Ab01175-23.0 Anti-Sonic Hedgehog [5E1]
Rat, Human
Rabbit IgG
Purified
In Stock

UniProt Accession Number of Target Protein: Q63673

Alternative Name(s) of Target: shh; sonic hh; Q15465
Immunogen: This antibody was raised by immunizing mice with purified rat SHH amino-terminal domain protein.

Specificity: This antibody is specific for the amino-terminal domain of rat SHH (SHH-N). It cross-reacts with the two other mammalian hedgehog ligands, Indian Hedgehog and Desert Hedgehog.

Application Notes: The binding specificity of this antibody for the amino-terminal fragment of SHH (SHH-N) has been confirmed in ELISA and Western blot analysis (Ericson et al, 1996).This antibody has been used to detect SHH in IHC analysis (Ericson et al, 1996).This antibody is able to inhibit hedgehog signalling, as it blocks the binding of all three mammalian Hh ligands to Ptc1 with low nanomolar affinity (Bosanac et al, 2009). In vivo, this inhibition is responsible for the observed growth retardation of HT55 an HT-29 xenografts in mice tumour models (Yauch et al, 2008). A Fab fragment derived from this antibody has been used in X ray crystallography analysis, in complex with human SHH (Maun, 2010).

Antibody first published in: Ericson et al. Two Critical Periods of Sonic Hedgehog Signaling Required for the Specification of Motor Neuron Identity Cell, Volume 87, Issue 4, 15 November 1996, Pages 661-673 PMID:8929535
Note on publication: Describes the original generation of this antibody, and its characterisation in ELISA, WB and IHC analysis.

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