United Kingdom (UK)
Recombinant monoclonal antibody to Sporozoite of Eimeria acervulina. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the chicken B-cell clone 6D-12-G10.
UniProt Accession Number of Target Protein: Q24769
Alternative Name(s) of Target: Sporozoite surface antigen; Eimeria acervulina
Immunogen: The original antibody was generated by immunizing chicken with sporozoite antigen of Eimeria acervulina, which is a 20-21 kDa protein.
Specificity: This antibody recognizes and binds the apical complex of the sporozoite of Eimeria acervulina, which is a 20-21kDa protein.
Application Notes: Avain coccidiosis is caused by an intracellulae protozoan belonging to the genus Eimeria. This disease is important in aviculture due to its negative effects on production and development of birds. Avian coccidiosis is one of the main causes of growth retardation, poor feed conversion and high mortality in intensive farming of broilers, cage-free pullets and breeders. This mAb recognizes the apical complex of the sporozoite in a western blot assay and ELISA. Immunoelectron microscopic examination revealed that the antibody stained the conoid antigen. In indirect immunofluorescence assay the culture supernatent containing the mAb intensely stained the tip of the E. acervulina sporozoites. It is also reported that this antibody inhibited the invasion of sporozoites in CD8+ T cells in vitro (PMID: 8627507). The scFv antibody was produced at a minimum of 7 mg l−1 and exhibited virtually identical antigen reactivity as the original mAb (Min et al., 2001). mAb 6D-12-G10 shows cross reactivities in immunofluorescence staining at the apical end of sporozoites of C. parvum and C. muris, and merozoites of C. parvum (PMID:15725532). This mAb is also cross reactive with other species like Apicomplexan parasites, including other Eimeria spp., Toxoplasma, Neospora, and Cryptosporidium spp (PMID: 27384177)
Antibody first published in:
Sasai et al. Characterization of a chicken monoclonal antibody that recognizes the apical complex of eimeria acervulina sporozoites and partially inhibits sporozoite invasion of cd8+ t lymphocytes in vitro. J Parasitol (1996); 82(1):82-7. PMID:8627507
Note on publication:
Describes the generation of this antibody and its use in identifying the Eimeria sporozoite antigen that binds to CD8+ lymphocytes.