United Kingdom (UK)
Recombinant monoclonal antibody to SSEA4. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma E2-F8.
Alternative Name(s) of Target: F8; Clone F8; Stage-specific embryonic antigen-4
Specificity: The antibody is specific for stage-specific embryonic antigen-4 (SSEA-4). SSEA-4 is a ganglioside which consists of a glycosphingolipid containing a terminal sialic acid residue (N-acetylneuraminic acid). SSEA-4 expression changes both qualitatively and quantitatively during development, differentiation and in tumorigenesis.
Application Notes: The specificity of the original format of the antibody was confirmed by ELISA analysis. The original format of the antibody was used for staining of fresh frozen human colon cancer tissue, demonstrating it could bind to individual cells and to discrete stromal components. The original format of the antibody was employed for lipid immunoblotting of a total lipid extract of HCT116 cells.
Antibody first published in: PMID:
Immunohistochemical staining of FFPE oesophageal adenocarcinoma tissue using anti-SSEA4 antibody E2-F8 (Ab04157). E2-F8 Anti-SSEA4 staining of FFPE oesophageal adenocarcinoma tissue using the chimeric rabbit version of E2-F8 (Ab04157-23.0). For staining of paraffin sections, antigen retrieval using a TrisEDTA buffer, pH 9, is recommended. A dilution range of 10-20 µg/ml is suggested, but users should titrate the antibody within their systems for best results. This antibody may be used to stain both frozen and paraffin-embedded tissue sections.
Data kindly provided by GlykoGen.