United Kingdom (UK)
Recombinant monoclonal antibody to Structural polyprotein. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma CHK152.
UniProt Accession Number of Target Protein: Q8JUX5; Q1H8W5; Q5XXP3
Alternative Name(s) of Target: p130
Immunogen: The original version of this antibody was raised by immunizing an adult C57BL/6 mice deficient for interferon regulatory factor 7 with La Reunion 2006 OPY-1 strain of CHIKV.
Specificity: This antibody is specific for the wings of the A domain on E2 of CHIKV.
Application Notes: The structure of the original version of this antibody was determined using cryo-EM. Further the original version of this antibody was used for a neutralization assay on CHIKV and Vero cells (Sun et al., 2013; PMID:23577234) (Pal et al., 2013; PMID:23637602) (Pal et al., 2014; PMID:24829346). The original version of this antibody was used for a prophylaxis study on immunodeficient Ifnar−/− C57BL/6 mice, which were infected with CHIKV (Pal et al., 2013; PMID:23637602) (Pal et al., 2014; PMID:24829346). This antibody was used for an ELISA on different CHIKV virions (Pal et al., 2013; PMID:23637602). The ability of this antibody to reduce viral infection in rhesus macaques was assessed. It showed that it was indeed able to reduce the viral load in these animals (Pal et al., 2014; PMID:24829346). This antibody has shown its ability to block access to CHKV virions and to block the E2 surface glycoprotein from enzymatic cleavage by trypsin (Blijleven et al., 2022; PMID:35215863).
Antibody first published in:
Pal et al. Development of a highly protective combination monoclonal antibody therapy against Chikungunya virus PLoS Pathog. 2013;9(4):e1003312. PMID:23637602
Note on publication:
The original paper screened 230 new mouse anti-CHIKV monoclonal antibodies (MAbs) for their ability to inhibit infection of all three CHIKV genotypes.