United Kingdom (UK)
Recombinant monoclonal antibody to Tenuazonic acid. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody 3F9.
Alternative Name(s) of Target: Nb-3F9; TeA; Alternaria mycotoxin tenuazonic acid; (5S)-3-Acetyl-5-[(2S)-butan-2-yl]-4-hydroxy-1,5-dihydro-2H-pyrrol-2-one
Immunogen: The original antibody was generated by immunizing a male Bactrian camel with TeA-CMO-KLH followed by antibody library construction and phage display-based selection.
Specificity: This antibody is specific for tenuazonic acid, an Alternaria mycotoxin. The antibody does not cross-react with AOH, AME, DON, ITeA, or ZEN.
Application Notes: The activity and sensitivity of the original format of this antibody (VHH) were assessed by ic-ELISA. Nb-3F9-Nluc, a biofunctional nanobody fusion, was generated and its nanoluciferase catalytic activity, nanobody binding activity, and specific TeA inhibition activity were analyzed. The performance of Nb-3F9-Nluc and the original Nb-3F9 was evaluated using two-step ELISA with HRP-anti-6×His tag secondary antibody. The IC50 value of ELISA based on Nb-3F9-Nluc was 80.3 ng/mL, and that for Nb-3F9 was 89.5 ng/mL. Additionally, Nb-3F9 and Nb-3F9-Nluc were used, respectively, in the development and optimization of chemiluminescent enzyme immunoassay and bioluminescent enzyme immunoassay (Wang et al., 2020; PMID: 32702970). TeA is a tetrameric acid derivative, and it is considered to have the highest toxicity and exposure among the Alternaria toxins. The contamination of TeA in foods has widely been a concern for food safety control.
Antibody first published in:
Wang et al. Chemiluminescent Enzyme Immunoassay and Bioluminescent Enzyme Immunoassay for Tenuazonic Acid Mycotoxin by Exploitation of Nanobody and Nanobody-Nanoluciferase Fusion Anal Chem. 2020 Sep 1;92(17):11935-11942. doi: 10.1021/acs.analchem.0c02338 PMID:32702970
Note on publication:
The original publication describes the development of highly specific nanobodies (Nbs), particularly Nb-3F9, against the Alternaria mycotoxin tenuazonic acid (TeA) using a stringent biopanning strategy.