United Kingdom (UK)
Recombinant monoclonal antibody to Tissue factor. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the synthetic clone hTFFab148H.
UniProt Accession Number of Target Protein: P13726
Alternative Name(s) of Target: CD142; TF; hTF; F3; Coagulation factor III; Thromboplastin; platelet tissue factor; factor III
Immunogen: The original antibody was isolated from a human Fab antibody library generated from peripheral blood collected from 300 healthy individuals.
Specificity: This antibody binds human tissue factor (TF) a hemostatic protein which is involved in the extrinsic pathway of coagulation. TF is receptor for circulating serine protease factor VIIa, activating the catalytic VIIa protease domain. The complex formed functions to activate the zymogen factor X into activated factor Xa, which then catalyzes the formation of Prothrombin to Thrombin. The pathways results in a fibrin clot which acts to stop bleeding. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Application Notes: The binding characterization of this Fab antibody was done using ELISA and dot blot. This antibody shows positive plasma coagulation inhibitory activity. This antibody is capable of neutralizing human tissue factor in vitro by inhibiting the binding of TF factor/Vlla complex to factor X. The inhibition rate increase with the concentration. The pharmacodynamic and pharmacokinetic activity of this antibody was studied in vivo in rat models (CN101423552).
Antibody first published in: PMID: