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- Ab02605-3.0 Anti-TL1A [chTL1A-1]
- Chicken
- Mouse IgG2b
- Purified
- In Stock
- Ab02605-3.3 Anti-TL1A [chTL1A-1]
- Chicken
- Mouse IgG2b
- Fc Silent™
- Purified
- Ships in 4-5 weeks
- Ab02605-23.0 Anti-TL1A [chTL1A-1]
- Chicken
- Rabbit IgG
- Purified
- In Stock
Recombinant monoclonal antibody to TL1A. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybrodoma chTL1A-1.
UniProt Accession Number of Target Protein: Q50L61
Alternative Name(s) of Target: TNFSF15; TNF-like ligand 1A; tumor necrosis factor superfamily 15; chicken TL1A; Tumor necrosis factor-like ligand 1A; chTL1A; TL1A mAb-1
Immunogen: The original antibody was raised by immunizing BALB/c mice with recombinant TL1A protein.
Specificity: The antibody binds specifically to chicken TL1A, a type II transmembrane protein predominantly expressed by endothelial cells that promotes the expansion of activated T cells and regulatory T cells, modulates inflammation, and regulates the production of a wide variety of T cell cytokines. The antibody does not react with the chIL-8/pET32a or proteinase K-pretreated chTL1A, verifying its specificity for the intact chTL1A.
Application Notes: The specificity of the original antibody was confirmed by ELISA analysis (Lee et al, 2014; pmid: 24565040). The antibody was used as a capture antibody in capture ELISA assay to measure serum TL1A levels in normal and necrotic enteritis (NE)-afflicted chickens. The antibody detected the recombinant chicken TL1A by western blot analysis. The antibody identified TL1A-secreting cells in the chicken thymus, cecal tonsil, and bursa of Fabricius by immunocytochemistry. Furthermore, the antibody inhibited chTL1A induced spleen lymphocyte proliferation, nitric oxide production by chicken macrophage cells (HD11), and blocked the cytotoxic effect of chTL1A against lymphoblastoid chicken B tumor cells (LSCC-RP9) (Lee et al, 2014; pmid: 24565040).
Antibody first published in:
Lee et al. Development and characterization of mouse monoclonal antibodies reactive with chicken TL1A. Vet Immunol Immunopathol. 2014;159(1-2):103-9. PMID:24565040
Note on publication:
The paper describes the generation and characterization of the antibody, specific for chicken TL1A.
Immunofluorescence staining of chicken blood leucocytes with anti-TL1A antibody chTL1A-1 (Ab02605). Immunofluorescence analysis of paraformaldehyde-fixed chicken blood leucocytes on Shi-fix™ coverslips stained with the mouse IgG2b version of chTL1A-1 (Ab02605-3.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, Ab02605-3.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-3.0) followed by staining with Alexa Fluor® 488 secondary antibody.