United Kingdom (UK)
Recombinant monoclonal antibody to TLR7. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma LTM3.
UniProt Accession Number of Target Protein: Q9NYK1
Alternative Name(s) of Target: Toll-like receptor 7; UNQ248/PRO285
Immunogen: The original antibody was generated by immunizing TLR7 deficient BALB/mice with Ba/F3 cell line expressing hTLR7-Flag-Hisx6/hUnc93B1-HAx2 mixed with a suitable adjuvant.
Specificity: This antibody binds human toll-like receptor 7 (TLR7). It also cross reacts with monkey TLR7. It is an endosomal receptor that plays a key role in innate and adaptive immunity. It also controls host immune response against pathogens through recognition of uridine-containing single strand RNAs (ssRNAs) of viral origin or guanosine analogs. TLR7 is considered to be a useful marker in indicating pathological condition of autoimmune diseases.
Application Notes: This antibody was used in combination with another anti-TLR7 antibody called rE3 to detect human TLR7 in a sandwich ELISA. LTM3 was used as used as a capturing antibody and biotinylated rE3 antibody (rE3-bio) was used a detecting antibody. This antibody can be used for the detection of human TLR7 in serum or plasma derived from human peripheral blood. The binding ability of this antibody for Ba/F3 cell line expressing human TLR7, mouse TLR7 and monkey TLR7 was checked using flow cytometry. This antibody can successfully detect human and monkey TLR7, but not mouse TLR7 in a flow cytometry (US20210199667).
Antibody first published in: PMID:
Immunofluorescence staining of Daudi cells with anti-TLR7 antibody LTM3 (Ab03265). Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of LTM3 (Ab03265-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, Ab03265-23.0, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.