United Kingdom (UK)
Recombinant monoclonal antibody to Tobacco mosaic virus. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma Mab24.
Alternative Name(s) of Target: TMV; neotope; intact TMV virions; intact birus; Nicotiana tabacum; rAb24
Specificity: This antibody recognizes a neotope on the surface of intact Tobacco mosaic virus (TMV) particles. TMV is a positive-sense single-stranded RNA virus species in the genus Tobamovirus that infects a wide range of plants, especially tobacco. Symptoms induced by Tobacco mosaic virus (TMV) are somewhat dependent on the host plant and can include mosaic, mottling, necrosis, stunting, leaf curling, and yellowing of plant tissues.
Application Notes: Infection of transgenic F1 plants producing mAb24-P with TMV showed a reduction of necrotic lesion numbers which is correlated with the amount of antibody produced in transgenic plants. This antibody is capable of inhibiting the infectivity of the virus if TMV is adsorbed in vitro at ratios as low as 300 antibody molecules per virion prior to inoculation (Voss et al., 1995). This antibody was used in the generation of a bispecific antibody biscFv2429 in transgenic suspension culture cells and tobacco plants, wherein two antibodies scFv24 and scFv29 were connected by the Trichoderma reesi cellobiohydrolase I linker. These antibodies bind two different epitopes of TMV. The epitope specificity of this antibody was determined using ELISA (PMID: 10411643). This antibody was used for the magnetic immunodetection of TMV (PMID: 25710366). This antibody was also used in the generation of recombinant fusion proteins scFv24-PDGFR and scFv24-TcRβ in transgenic tobacco suspension cultures and transgenic plants. Bioassays of viral infection showed that transgenic tobacco plants expressing scFv24-TcRβ were resistant to TMV infection (Schillberg et al., 2000).
Antibody first published in:
Fischer et al. Affinity-purification of a TMV-specific recombinant full-size antibody from a transgenic tobacco suspension culture. J Immunol Methods. 1999 Jun 24;226(1-2):1-10. PMID:10410966
Note on publication:
Describes the expression and purification of a recombinant anti-TMV antibody in a transgenic tobacco plant.