Anti-UBE2C [SAIC-41C-6]

Description:

Recombinant monoclonal antibody to UBE2C. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the B-cell cDNA library SAIC-41C-6.

Clone Name:

SAIC-41C-6

Published Application(s):

immunoMRM, WB

Published Species Reactivity:

Human

Products based on this clone:

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Ab00334-23.0 Anti-UBE2C [SAIC-41C-6]
Human
Rabbit IgG
Purified
In Stock

Ab00334-1.1 Anti-UBE2C [SAIC-41C-6]
Human
Mouse IgG1
Purified
Ships in 4-5 weeks

UniProt Accession Number of Target Protein: O00762

Alternative Name(s) of Target: Ubiquitin-conjugating enzyme E2 C; UBCH10
Immunogen: Peptide "LSLEFPSGYPYNAPTVK" derived from the Ubiquitin-conjugating enzyme E2C, conjugated to KLH.

Specificity: Recognises human UBE2C.

Application Notes: Original data characterizing this antibody may be found <a href="http://antibodies.cancer.gov/apps/site/detail/Serpin%20Peptidase%20Inhibitor%2C%20Clade%20A%20(Alpha-1%20Antiproteinase%2C%20Antitrypsin)%20Member%206%20Peptide%201#Serpin_Peptidase_Inhibito

Antibody first published in: Schoenherr et al. Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Mol Cell Proteomics PMID:25512614
Note on publication: Describes generation a panel of recombinant antibodies using synthetic peptides of human proteins as well as validation using ELISA and multiple reaction monitoring-mass spectrometry.

Flow cytometry analysis of MCF7 cells using Ab00334-23.0. Click on image for more details.

Flow cytometry using the anti-UBE2C antibody SAIC-41C-6 (Ab00334). Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of SAIC-41C-6 (Ab00334-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

IF staining of Caco-2 cells using Ab00334-23.0. Click on image for more details.

Immunofluorescence staining of Caco-2 cells with anti-UBE2C (Ab00334) SAIC-41C-6 Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-41C-6 (Ab00334-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00334-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Flow cytometry analysis of MCF7 cells using Ab00334-23.0. Click on image for more details.