Anti-UBE2I [SAIC-36A-9]

Description:

Recombinant monoclonal antibody to UBE2I. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the B-cell cDNA library SAIC-36A-9.

Clone Name:

SAIC-36A-9

Published Application(s):

immunoMRM, WB, ELISA

Published Species Reactivity:

Human

Products based on this clone:

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Ab00329-23.0 Anti-UBE2I [SAIC-36A-9]
Human
Rabbit IgG
Purified
In Stock

Ab00329-1.1 Anti-UBE2I [SAIC-36A-9]
Human
Mouse IgG1
Purified
In Stock

UniProt Accession Number of Target Protein: P63279

Alternative Name(s) of Target: SUMO-conjugating enzyme UBC9; UBC9; UBCE9
Immunogen: Peptide "GLFIIDGK", derived from the SUMO-conjugating enzyme UBC9, conjugated to KLH.

Specificity: Recognises human UBE2I.

Application Notes: Original data characterizing this antibody may be found here .

Antibody first published in: Schoenherr et al. Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Mol Cell Proteomics PMID:25512614
Note on publication: Describes generation a panel of recombinant antibodies using synthetic peptides of human proteins as well as validation using ELISA and multiple reaction monitoring-mass spectrometry.

Flow cytometry analysis of Jurkat cells using Ab00329-23.0. Click on image for more details.

Flow cytometry using the Anti-UBE2I antibody SAIC-36A-9 (Ab00329). Paraformaldehyde fixed Jurkat cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of SAIC-36A-9 (Ab00329-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

IF staining of Jurak cells using Ab00329-23.0. Click on image for more details.

Immunofluorescence staining of Jurak cells with anti-UBE2I (Ab00329) SAIC-36A-9 Immunofluorescence analysis of paraformaldehyde fixed Jurak cells on Shi-fix™ cover-slips stained with the chimeric rabbit IgG version of SAIC-36A-9 (Ab00329-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab00329-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.