- Ab03726-23.0 Anti-VP28 [Mab 216]
- White spot syndrome virus (WSSV)
- Rabbit IgG
- Ships in 5-6 weeks
Recombinant monoclonal antibody to VP28. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma Mab 216.
UniProt Accession Number of Target Protein: Q9ICB7
Alternative Name(s) of Target: WSSV; WSSV VP28; 25kDa structural protein VP25; Envelope protein VP28; Major structural protein VP28; Mab216; 216
Immunogen: The original antibody was generated by immunizing female WKY/NCrj rats with crude virus preparations and hybridomas were generated using cells from the enlarged medial iliac lymph nodes.
Specificity: This antibody binds the VP28 envelope protein of the White spot syndrome virus (WSSV). White spot syndrome virus (WSSV) is a major pathogen in shrimp aquaculture. VP28 is one of the most important envelope proteins of WSSV.
Application Notes: This antibody was capable of detecting VP28 protein of WSSV in a western blot. Immuno-electron microscopy further showed that the epitope of this antibody is located on the viral envelope protein. In vitro neutralization studies in shrimp primary lymphoid cell culture showed diminished infectivity. In vivo neutralization assay showed 80% survival in shrimps injected with WSSV at a dilution of 1 × 10−3 pre-incubated with purified immunoglobulin (IgG) version of MAb 216. At higher virus concentration this antibody was not able to neutralize the virus (K.D.T. Natividad et al., 2007).
Antibody first published in:
K.D.T. Natividad et al. White spot syndrome virus (WSSV) inactivation in Penaeus
japonicus using purified monoclonal antibody targeting
viral envelope protein. Aquaculture 269 (2007) 54–62. PMID:
Note on publication: This paper describes the generation and characterization of a monoclonal antibody Mab 216 and evaluates its neutralizing activity both in vivo and in vitro